Selection of a compound with the best in vivo activity against a tumor cell line of interest compound screening identification of the most suitable cell line for an in vivo efficacy study with a selected test compound tumor model screening. Syngas mass transfer analysis in a hollow fiber reactor. This article provides a strategy for specifying operating conditions for the system based on mathematical models of oxygen delivery to the cell population. Hydrolysis of butteroil by immobilized lipase using a hollow. Pdf catalytic hollow fibre based reactors for a enhance. Vibration of hollow fibre membranes in submerged membrane bio reactors smbrs li tian school of civil and environmental engineering a thesis submitted to the nanyang technological university. Pdf flat sheet or hollow fibre comparison of fullscale. Experiments using single fibers have been conducted to obtain conversion data for reactor scaleup. May 26, 2016 this manuscript describes in detail the setup and operation of a hollow fiber bioreactor system for the in vivolike culture of mammalian cells.
Inorganic hollow fibre membranes in catalytic processing. In this study, we report the development of a microbore hollow fiber enzyme reactor mhfer coupled to nanoflow liquid chromatographytandem mass spectrometry nlcesimsms for the online digestion or selective enrichment of glycopeptides and analysis of proteins. Pdf hollow fiber bioreactors are the focus of scientific research aiming to mimic physiological vascular networks and engineer organs and tissues in. For adherent cell types, hfbs offer an in vivolike microenvironment as each fibre replicates a blood capillary and the mass transfer rate across the wall is independent from the shear stresses experienced by the cell. A hollow fiber bioreactor is a 3 dimensional cell culturing system based on hollow fibers, which are small, semipermeable capillary membranes arranged in parallel array with a typical molecular weight cutoff mwco range of 1030 kda. A novel hollow fiber membrane reactor for high purity h 2 generation from thermal catalytic nh 3 decomposition miao yu pi, rensselaer polytechnic institute shiguang li co. These hollow fiber membranes are often bundled and housed within tubular polycarbonate shells to create hollow fiber bioreactor cartridges. Selection of a compound with the best in vivo activity against a tumor cell line of interest compound screening. Hollow fiber reactors have been developed for many biochemical and biomedical applications. A side view of a series of fibers mounted in the immp reactor. Catalytic hollow fibre membrane reactors for h production. In vivo hollow fiber model proqinase drug discovery in. Advances in fiber construction and methods for oxygenation have the potential to bring the advantages of hollow fiber cell culture to biomanufacturing.
Production of exosomes in a hollow fiber bioreactor. Ethanol production in isotropic hollow fiber membranebioreactors. Doran, in bioprocess engineering principles second edition, 20. Mar 15, 2007 hollow fiber reactors have been developed for many biochemical and biomedical applications. Hollow fibre modules typically contain 50 to 5000 selfsupporting, narrowbore membrane tubes, as shown schematically in figure 11. Hollow fiber reactors reduce cost, scaleup concerns. This thesis focuses on the development of a catalytic hollow fibre. A 30l hollow fibre reactor with continuous fermentation for cell recycling of escherichia coli as 1.
Hollow fiber reactors reduce cost, scaleup concerns by angelo depalma pharmaceutical manufacturers producing up to about 100 kgyr of therapeutic or diagnostic antibodies may soon replace roller bottles, ascites, stirred tanks, and airlift fermenters with hollow fiber bioreactors. Hollow fiber bioreactor technology for tissue engineering. Figure 1 illustrates the flow pattern and pressure distribution in the hollow fiber. The composite membrane consisted of a porous support layer covered with a thin dense film, which was responsible for oxygen enrichment of the permeate stream. As a module, hollow fiber membranes are packed together to form a bundle which is then placed in a shell and sealed at both ends. Design, construction, modelling and control of a dualhollow. Department of chemical engineering and materials science, university of minnesota, minneapolis, mn 55455. Hollow fiber postcolumn reactor for liquid chromatography.
Alphagalactosidase production and use in a hollowfiber. Li department of chemical engineering, imperial college london, london sw7 2az, united. Diffusive oxygen transport from the fiber wall to the. Feed enters at one end of the module and retentate leaves at. The pores of the sponge structure serve as a housing for microorganisms or cells with high.
The hollowfiber membrane systems have been used as bioreactors years ago due to the. Development of an online microbore hollow fiber enzyme. Mass transfer limitations in diffusionlimited isotropic hollow fiber bioreactors. Ethanol production saccharomyces cerevisiae immobilized. Operating diagrams are presented that describe reactor performance as a function of important operating variables like oxygen concentration and the fiber packing. Pdf catalytic hollow fibre based reactors for a enhance h2. Thereactor shells ofc20andc40 were inoculated simultaneously with an exponentialphase yeast inoculum107 cells perml. View the article pdf and any associated supplements and figures for a period of 48 hours.
Work on enzymes, animal cells and plant cells will be men tioned only where the findings are relevant to microbial cell culture. Graphical representation of tissue engineering using hollow fibers as scaffolds. Catalytic hollow fibre based reactors for a enhance h2 production by methanol steam reforming. Abstract a scalable flow reactor is demonstrated for enantioselective and regioselective rhodium carbene reactions. The in vivo hollow fiber model is a fast and economic in vivo screening approach which can be used for two different purposes. Hollow fibre membrane arrays for co 2 delivery in microalgae photobioreactors. Design and operating criteria for hollow fiber bioreactors. In the study of these reactor systems, we have used single fiber reactors as a prototype for the larger hollow fiber cartridges. A hollow fiber bioreactor is a 3 dimensional cell culturing system based on hollow fibers, which. The hff is composed of around one hundred modules each of which contains several thousand hollow fibers soaking in unprocessed water. Hollow fibre membrane arrays for co2 delivery in microalgae. Declaration of originality i hereby declare that the work reported in this thesis was composed and originated entirely by me. Microstructured catalytic hollow fibre reactor for methane.
Asymmetric al 2 o 3 hollow fibres produced by a phaseinversion and sintering technique were employed as a single substrate for both coating of the pd membrane and impregnation of the 30%cuoceo 2 catalyst. Hollow fiber reactors allow scaleup without a proportional increase in production costs while providing economy of scale. Crud is trapped on the outer surface of the hollow fiber and effused water forms an internal flow directed toward the one open end. Hollow fiber bioreactors are the focus of scientific research aiming to mimic physiological vascular networks and engineer organs and tissues in. One method that can improve gas exchange within photobioreactors without excessive mixing is the use of hollow fibre membranes, which enable simultaneous transport of gases deep into the reactor and rapid exchange with the culture media. American type culture collection 53771 in a shakeflask reactor hydroxylated diclofenac 50. When enzymes are entrapped in the hollow fiber lumen, substrate mole cule can approach the enzyme directly, thus avoiding the reaction controlled by permeation. Fluorimetric determination of amino acids by highperformance liquid chromatography using a hollowfibre membrane reactor.
Production of exosomes in a hollow fiber bioreactor john j s cadwell, president and ceo, fibercell systems inc introduction exosomes are small lipid membrane vesicles 80120 nm of endocytic origin generated by fusion of cytoplasmic endosomal multivesicular bodies with the cell surface. The enzyme solution was recirculated with a peristaltic pump through. The potential application of hollow fiber bioreactors to. Unlike hollow fibers with fingerlike straight pores used in other works wang et al. Hollowfiber membrane an overview sciencedirect topics. This hollow fiber membrane without fingerlike pores could bring higher mechanical strength. Polymeric hollow fiber membranes for bioartificial organs and tissue. A strategy to determine operating parameters in tissue. These reactors offer a very gentle means for immobilizing biocatalysts such as enzymes, animal ceils, plant cells, or microbial cells. Amundson, hydrolysis of butteroil by immobilized lipase using a hollow.
Hollow fiber membrane life in membrane bioreactors mbr. Frontiers nitrogen production by efficiently removing. Soybean milk serves as a base for a variety of beverages designed for consumption in developing countries. An alternative approach is the use of hollow fiber cartridge hfc reactors. Pdf hollow fiber bioreactor technology for tissue engineering. Diffusive oxygen transport from the fiber wall to the cells can. Theinoculum was not drained fromthe reactor shell, and both shell ports ofeach reactor were closed for the duration of the experiment so that nutrients and.
Abstract mammalian cell culture in hollow fiber reactors is hampered by oxygen limitation. The hollow fibers were sealed on one end and were pressurized with hydrogen on the other end. Harvest large quantities of secreted protein with hollow. Catalytic hollow fibre membrane reactors for h2 production core. However, implementing smallscale hollow fiber bioreactors for culturing mammalian cells can save a lot of time and money, and is especially useful for harvesting large quantities of secreted protein. Jul 09, 2016 however, implementing smallscale hollow fiber bioreactors for culturing mammalian cells can save a lot of time and money, and is especially useful for harvesting large quantities of secreted protein. Interfacial microfluidic processing of metalorganic. Hollow fibre reactor definition and examples biology. Hollow fiber bioreactors for in vivolike mammalian tissue.
This thesis focuses on the development of a catalytic hollow fibre membrane reactor for the combined steam methane reforming smr and watergas shift wgs reaction, using a nibased catalyst, and at a temperature window suitable for harvesting pure h2, a clean energy carrier, from. Ideal for ultrafiltration, diafiltration and microfiltration, singleuse spectrum hollow fiber filters for tangential flow filtration are packed in housings engineered to handle high pressures, with a choice of fiber chemistries and sizes from process development to manufacturing. Biological cultures are most commonly performed in flasks, roller bottles or spinners. Schematic drawing of hollow fiber enzyme reactor from re. Asymmetric hollow fibers are employed in a flow reactor, where the hollow fibers have a semipermeable membrane surrounding a lumen, where the semipermeable membrane is supported by a sponge structure.
A novel hollow fiber membrane biofilm reactor hfmbr was developed to remove nitrate from contaminated drinking water using molecular hydrogen as a clean electrondonor substrate. A novel hollow fibre membrane biofilm reactor for autohydrogenotrophic denitrification of drinking water. Experimental and theoretical studies on a backflush hollow. This thesis focuses on the development of a catalytic hollow fibre membrane reactor for the combined steam methane reforming smr and watergas shift wgs reaction, using a nibased catalyst, and at a temperature window suitable for harvesting pure h2, a clean energy carrier, from the reaction by a pd membrane. This manuscript describes in detail the setup and operation of a hollow fiber bioreactor system for the in vivolike culture of mammalian cells. Development of a catalytic hollow fibre membrane micro. Hollow fiber bioreactors hollow fiber membrane modules have been used as bioreactors to pro duce or transform chemicals.
Burns doctor of philosophy university of edinburgh. Jul 27, 2014 hollow fibre membrane bioreactors hfb provide a novel approach towards tissue engineering applications in the field of regenerative medicine. This article describes development of a catalytic hollow fibre membrane micro reactor chfmmr for high purity h 2 production. Methods for microbiological processing of organic materials for production of valuable products. In this study, asymmetric al2o3 hollow fibres have been used as a support for either a cuzngaox based catalyst or a pdag membrane in the development of a catalytic hollow fibre micro reactor. Flat sheet or hollow fibre comparison of fullscale. Us4440853a microbiological methods using hollow fiber. Present and future in the biotechnology industry jose m. Hollow fibre membrane bioreactors for tissue engineering.
Hollow fibre membrane bioreactors hfb provide a novel approach towards tissue engineering applications in the field of regenerative medicine. Compact hollow fibre reactors for efficient methane conversion article pdf available in journal of the european ceramic society 3716 april 2017 with 20 reads how we measure reads. Pdf hollow fibre bioreactor for wastewater treatment using. Hollow fibre reactor a fermentation system in which the cells are separated from the medium using semipermeable membranes arranged in the form of hollow fibres. A novel hollow fiber membrane reactor for high purity h 2.
A fibercell systems hollow fibre bioreactor enables the continuous culture of large numbers of cells at high density. Flat sheet or hollow fibre comparison of fullscale membrane bio reactor configurations. Catalytic hollow fibre membrane reactors for h2 production. The shellandtube structure makes heat and mass transfer. A scalable flow reactor is demonstrated for enantioselective and regioselective rhodium carbene reactions cyclopropanation and c.
Fibercell systems hollow fibre bioreactor for high density. Abstract a highly permeable composite hollow fibre membrane developed for air separation was used in a membrane aerated biofilm reactor mabr. The hollow fiber bioreactor system delivers media to the cells in a manner akin to the delivery of blood through the capillary networks in vivo. H functionalization by developing cascade reaction methods employing a microfluidic flow reactor system containing immobilized dirhodium catalysts in conjunction with the flow synthesis of diazo compounds. Unlike conventional cell culture methods, the cost for hollow fiber production of one milligram decreases as production increases. Design, construction, modelling and control of a dual hollow fibre bioreactor for iybridoma cells john w. Pdf compact hollow fibre reactors for efficient methane. The complete description of the transport of fluid in hollow fiber capillary membrane requires that the local transport equations that describe the flow and transport conditions should be solved. Shuler, the measurement of effective substrate diffusities within whole cell suspensions using a diffusion. These reactors offer a very gentle means for immobilizing. Soybean flour contains raffinose and stachyose considered to be responsible for flatulence often associated with these products j. Effects of temperature, biotechnology and bioengineering, 39, 11, 10971111, 2004. The present invention is concerned with a hollow fibre membrane for use in a membrane supported biofilm reactor msbr or the like, the hollow fibre membrane comprising a substantially cylindrical sidewall defining an internal lumen from which gas can permeate through the sidewall, and characterised in that at least a part of an outer surface of the fibre membrane is engineered to define at.
Rabies pathobiology and its rna virus agent lyssavirus. Vicaria department of chemical engineering, faculty of sciences, university of granada, 18071 granada, spain and it is possible perform a simple scaling using several units in parallel form, obtaining high productivities. View enhanced pdf access article on wiley online library html view download pdf for offline viewing. A novel hollow fiber membrane reactor for high purity h 2 generation from thermal catalytic nh 3 decomposition miao yu pi, rensselaer polytechnic institute shiguang li copi, gas technology institute. Hollow fiber membrane bundles are widely used as liquidtoair contactors. The development of tissue engineering hollow fiber bioreactors hfb requires the optimal design of the geometry and operation parameters of the system. With mhfer, enzymatic digestion of protein could be achieved by continuous flow within a very small volume. Microstructured catalytic hollow fibre reactor for. Flat sheet or hollow fibre comparison of fullscale membrane bioreactor configurations. Wo20155977a1 an aerated biofilm reactor hollow fibre. Its a timescalable production process thats ideal for generating significant amounts of very concentrated evsexosomes, mabs or recombinant proteins.
Hollow fiber membrane modules have been used as bioreactors to pro duce or transform chemicals. Biosynthesis of drug metabolites using microbes in hollow. Here we focus on the use of hollow fiber bioreactors for the culture of microorganisms. Hollow fiber bioreactors have been in use for more than 30 years for the production of secreted proteins and antibodies from mammalian cells. A study of polynucleotide phosphorylase production by. Hollow fibre module an overview sciencedirect topics. The hollow fiber reactor hfr is one type of reactor that has the potential for achieving appropriate mass transfer rates for biofuels production, but a better understanding of mass transfer limitations associated with all syngas species is critical for assessing syngas usage during fermentation. Catalytic hollow fibre membrane reactors for h 2 production ana maria gouveia gil a thesis submitted for the degree of doctor of philosophy and the diploma of imperial college london february 2015. Pdf a laboratory scale hollow fibre membrane bioreactor which used bubbleless membrane aeration was tested over 5 months for its ability. Feed enters at one end of the module and retentate leaves at the other end. The performance of hfc reactors is compared with shakeflask reactors using diclofenac as a model substrate.
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